Technical Data
A1475-01H
AMP Activated Protein Kinase alpha1 (AMPK a1)
Description:
The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1) is a heterotrimeric protein comprising alpha (63kD), beta (38kD) and gamma (38kD) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl- CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:100-1:250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k21Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tri-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human AMPK alpha-1 subunit. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mitchelhill, K. I., et al. (1994) J. Biol. Chem. 269, 2361-2364. 2. Stapleton et al. J. Biol. Chem. 271:611-614. 3. Hamilton et al. FEBS Lett. 500:163-168, 2001 4. Carling, D., et al., (1989) Eur. J. Biochem. 186; 129-136.