		Technical Data
A1475-01H	AMP Activated Protein Kinase alpha1 (AMPK a1)

Description:
The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1) is a heterotrimeric protein comprising alpha (63kD), beta (38kD) and gamma (38kD) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl- CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:100-1:250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	6k21	Supernatant

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

Not Determined

Immunogen:

A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit.

Purity:

Supernatant

Form

Supplied as a liquid in 50mM Tri-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

Specificity:

Recognizes human AMPK alpha-1 subunit. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mitchelhill, K. I., et al. (1994) J. Biol. Chem. 269, 2361-2364. 2. Stapleton et al. J. Biol. Chem. 271:611-614. 3. Hamilton et al. FEBS Lett. 500:163-168, 2001 4. Carling, D., et al., (1989) Eur. J. Biochem. 186; 129-136.