Technical Data
A1580-03D
Amphiphysin (AMPH, AMPH1)
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Technical Data |
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A1580-03D |
Amphiphysin (AMPH, AMPH1) |
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Description: Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrinmediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2,000 Immunoprecipitation: 1-2ul/400ul lysates Immunohistochemistry: 1:100 Immunocytochemistry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. |
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