Technical Data
A1580-03D
Amphiphysin (AMPH, AMPH1)
Description:
Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrinmediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2,000
Immunoprecipitation: 1-2ul/400ul lysates
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
TypeIsotypeCloneGrade
MabIgG1,k10K105Ascites
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human GST-fragment protein (320-696AA) purified from E.coli.
Purity:
Ascites
Form
Supplied as a liquid in Hepes, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
Specificity:
Recognizes human Amphiphysin. Species crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang, B. and Zelhof, A.C. (2002) Traffic, 3:452-460, 2. Wigge, P. and McMahon, H.T. (1998) Trend:Neurosci. 21, 339-344.