Technical Data
A1590
Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin Precursor, AR, Colorectum Cell-derived Growth Factor, CRDGF, MGC13647, SDGF)
Description:
The mature secreted form Amphiregulin (AR) is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR expression is induced by phorbol ester, estrogen, androgen and other ligands of EGFR. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells and keratinocytes. AR is important in development of human placenta and murine mammary gland. AR is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (native verified): 10ug/mg protein lysate. Use Protein A.
Western Blot: 5ug/ml for 2hrs at RT.
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T and HT29 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic 19-mer peptide, corresponding to aa26-44 from secreted Amphiregulin of human origin. Cellular Localization: Cytoplasmic and/or nuclear. MW of Antigen: 18.5-22.5kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See A1590X.
Specificity:
Recognizes human Amphiregulin. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Johnson, G.R., et al., J. Cell Biol. 118: 741-751 (1992). 2. Johnson, G.R., et al., J. Biol. Chem. 268: 18,835-18,843 (1993). 3. Johnson, G.R. & Wong, L., J. Biol. Chem. 269: 27,149-27,154 (1994). 4. Martinez-Lacaci, I., et al., Endocrinology 136: 3983-3992 (1995). 5. Schuger, L., et al., Development 122: 1759-1767 (1996).