Technical Data
A2275-60A2
Amyloid A, Serum (Serum Amyloid A, SAA)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (frozen tissue and paraffin sections): 1:10; Incubation Time 1 hr ,at RT
Note: no proteolytic treatment required
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5F300Supernatant
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human serum amyloid A (APO SAA).
Purity:
Culture supernatant
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes Human Amyloid A, Serum. Useful for the detection of AA-type amyloid and for the discrimination from other type of amyloid (AL-type and b2M-amyloid) in human tissue biopsies. Reacts with amyloid depositions in all organs and tissues. Recommended for Renal biopsy with amyloid depositions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linke RP, Nathrath WBJ, Eulitz M. Classification of amyloid syndromes from tissue sections using antibodies against various amyloid fibril proteins: report of 142 cases. In: Glenner CG, Osserman EF, Benditt EP, Calkins E, Cohen AS, Zucker-Franklin D, eds. Amyloidosis. Amsterdam, Pneunum Publishing Corporation (1986):599-605. 2. Linke RP, Huhn D. Ultrastructural identification of AA-type amyloid fibrils using polyclonal and monoclonal antibodies. In: Glenner CG, Osserman EF, Benditt EP, Calkins E, Cohen AS, Zucker-Franklin D, etds. Amyloidosis. Amsterdam, Plenum Publishing Corporation (1986): 79-85.