Immunohistology (Formalin/paraffin): (Use Ab at 1:30-1:60 for 30min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control: Amyloid deposits in kidney.
Cellular Localization: Extracellular
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Human amyloid A.|
|Supplied as a liquid with 0.09% sodium azide.|
|Reacts with isolated protein AA in both native fibril form and denatured form. It reacts with amyloid deposits in all organs and tissues including kidney and rectum, and as well as the serum precursor of protein AA. Species Crossreactivity: Human.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Linke RP. Identification of amyloid protein AA with a monoclonal antibody. Blut 1982;45:407-9. 2. Linke RP. Monoclonal antibodies against fibril protein AA. Production, specificity, and use for immunohistochemical localization and classification of AA-type amyloidosis. J Histochem Cytochem 1984;32:322-8. 3. Linke RP. Identification of AA-type amyloid in tissue sections using monoclonal antibodies. In: Peeters H, ed. Protides Biol Fluids. Oxford; Pergamon Press 1983;31:835-8.|