Technical Data
A2275-61B
Amyloid A Component
Description:
Amyloid A is a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60-promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. It also acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. The protein is expressed in all fetal tissues, with highest levels in brain, kidney, heart and spleen. Multiple transcript variants encoding ten different isoforms have been found for the gene encoding Amyloid A protein. Mutations in the gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.

Positive Control:
Amyloid deposits in kidney.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k5G6Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human amyloid A. Cellular Localization: Extracellular
Purity:
Tissue culture supernatant
Form
Supplied as a liquid, 0.09% sodium azide
Specificity:
Recognizes isolated human protein A in both native fibril form and denatured form. It also recognizes amyloid deposits in all organs and tissues including kidney and rectum, as well as the serum precursor of protein A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linke RP. Identification of amyloid protein AA with a monoclonal antibody. Blut 1982;45:407-9. 2. Linke RP. Monoclonal antibodies against fibril protein AA. Production, specificity, and use for immunohistochemical localization and classification of AA-type amyloidosis. J Histochem Cytochem 1984;32:322-8. 3. Linke RP. Identification of AA-type amyloid in tissue sections using monoclonal antibodies. In: Peeters H, ed. Protides Biol Fluids. Oxford; Pergamon Press 1983;31:835-8.