Technical Data
A2275-69R1
Amyloid Precursor Protein, Control Peptide (APP, Amyloid beta (A4) Precursor Protein)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for A2275-69R, Amyloid Precursor Protein, polyclonal antibody.

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42, and 1-43; approx. 4.5kD). Polymerization of bamyloid Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.

APP gene has 19 exons. At least 8 different types of APP (APP695, APP751, and APP770 are the major ones) are produced as a result of alternative splicing of exons 7, 8, and 15. Exons 7 codes for a 57aa domain that is analogous to Kunitz protease inhibitors (KPI). Exon 8 codes for a 19aa residue domain with homology to the MRC OX-2 antigen found on the membrane of neurons, while exon 15 codes for an 18aa domain, 16aa proximal to the Ab region. APP695 lacks exons 7 and 8, whereas APP770 and APP751 represents the full length and the exon 8 lacking isoform. Beta amyloid A4 protein represents 597-638aa of APP695 28aa from the extracellular domain and 14-15aa from the extracellular domain). APP695 is predominantly expressed in the brain while APP751 and APP770 are also found in other tissues.

Source:
Synthetic peptide corresponding to 20aa of human APP695. Epitope location: N-terminal, Extracellular

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 5-10ug
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
~3kD
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized pwder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kang J et al (1987) Nature 325, 733. 2. Kang J et al (1989) Nucleic Aacid Res. 17, 2130. 3. Shivers BD et al (1988) EMBO J. 7. 1365-1370. 4. Ponte P et al (1988) Nature 3, 31, 525-527. 5. Schilling J et al (1991) Gene 98, 225-230. 6. Yoshikai S et al (1987) Gene 87, 257-263. 7. Neve R et al (1992) PNAS 89, 3448. 8. Goldgaber D et al (1987)Science 235, 877. 9. Yankner B et al (1989) Science 245, 417. 10. Golde T et al (1992) Science 255, 728.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.