Technical Data
A2275-77
b-Amyloid Peptide
|
Technical Data |
|
A2275-77 |
b-Amyloid Peptide |
|
|
Description: The progressive deposition of insoluble amyloid protein plaques in speci c areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease. The major component of these protein plaques is a peptide of 39–42 amino acids derived from a group of 695–770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well de ned. In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly de ned. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (alcohol-fixed, parrafin embedded, frozen): ~10ug/ml It is nessessary to pretreat the tissue with trypsin digestion. Western Blot: 0.1-0.2ug/ml ELISA: 0.5-1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||