		Technical Data
A2293	Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

Description:
Tie-1 (human 1138aa; mouse 1134aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Murine and human Ang-1 are 498aa secreted angiogenic proteins. It is only 60% homologous with Ang-2. Ang-1 is the natural ligand for Tie-2 and is primarily expressed in developing endothelial cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 (50-100ng of control peptide/
well)
Western Blot: 1:1000-1:5000 (Expected size of Ang-1 is ~55kD)
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
A2293-05: Angiopoietin 1, Control Peptide (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to 20aa near the N-terminus of the mouse Ang-1 (KLH). Species Sequence Homology: Human: 90%; porcine, rat, bovine: 95%.

Purity:

Neat serum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes mouse Ang-1 (~55kD). Detects ~70kD glycosylated, secreted protein in the conditioned media from cells expressing recombinant Ang-1. No significant sequence homology with Ang-2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Davis, S., et al., Cell 87: 1161-1169 (1996). 2. Nomura, N., et al., DNA Res. 1: 27-35 (1994). 3. Suri, S., et al., Cell 87: 1171 (1996). 4. Maisonpierre, C., et al., Science 277: 55 (1997).