Technical Data
A2293
Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)
Description:
Tie-1 (human 1138aa; mouse 1134aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Murine and human Ang-1 are 498aa secreted angiogenic proteins. It is only 60% homologous with Ang-2. Ang-1 is the natural ligand for Tie-2 and is primarily expressed in developing endothelial cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 (50-100ng of control peptide/
well)
Western Blot: 1:1000-1:5000 (Expected size of Ang-1 is ~55kD)
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
A2293-05: Angiopoietin 1, Control Peptide (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to 20aa near the N-terminus of the mouse Ang-1 (KLH). Species Sequence Homology: Human: 90%; porcine, rat, bovine: 95%.
Purity:
Neat serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes mouse Ang-1 (~55kD). Detects ~70kD glycosylated, secreted protein in the conditioned media from cells expressing recombinant Ang-1. No significant sequence homology with Ang-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Davis, S., et al., Cell 87: 1161-1169 (1996). 2. Nomura, N., et al., DNA Res. 1: 27-35 (1994). 3. Suri, S., et al., Cell 87: 1171 (1996). 4. Maisonpierre, C., et al., Science 277: 55 (1997).