Technical Data
A2293-04
Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)
Description:
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis and it accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Angiopoietin 1 (mouse and human: 498aa; ~98% identity) is an angiogenic secreted protein that interact with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Angiopoietin 1 binds and induces tyrosine phosphorylation of Tie-2. Angiopoietin 1 appears to play a crucial role in mediating matrix and mesenchyme.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 (50-100ng control peptide/well)
Western Blot: 1-10ug/ml (ECL)
Immunohistochemistry: 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2293-05: Angiopoietin 1, Control Peptide

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 20aa near the N-terminus of the mouse Ang-1 conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes mouse Ang-1 at ~55kD. May recognize glycosylated, secreted protein in the conditioned media from cells expressing recombinant Ang-1 at ~70kD. No significant sequence homology with Ang-2. Species Sequence Homology: human: 90%; porcine, rat, bovine: 95%>
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Johansson, A. et al., (2005) Endocrinology 146:3463-3470. 1. Davis, S., et al., Cell 87: 1161-1169 (1996). 2. Nomura, N., et al., DNA Res. 1: 27-35 (1994). 3. Suri, S., et al., Cell 87: 1171 (1996). 4. Maisonpierre, C., et al., Science 277: 55 (1997).