Technical Data
Angiopoietin-1, Control Peptide (Ang-1, Angpt1, Agpt)
Molecular Biology Storage: -20CShipping: Blue Ice
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Study of tumor angiogenesis has led to the identification of several proteins in angiogenic pathway.

Angiopoietin-1 (mouse and human Ang-1; 498aa; ~98% identity) is an angiogenic secreted protein that interact with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 mouse and human Ang-2, 496aa; ~85% identity) has recently been identified. It may act an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.

Synthetic peptide corresponding to 20aa of mouse Ang1, N-terminal.

Molecular Weight:

Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Synthetic peptide, mouse Ang-1
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized pwder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Davis S et al (1996) Cell 87, 1161-1169. 2. Nomura N et al (1994) DNA Res. 1, 27-35. 3. Suri, S et al (1996) 87, 1171. 4. Davis, S et al (1996) cell 87, 1161. 5. Maisonpierre C et al (1997) Science 277, 55.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.