Technical Data
A2293-10
Angiopoietin 2 (Ang-2, AGPT2, ANGPT2, Ang2)
Description:
Angiopoietin-1 is an angiogenic secreted protein that interacts with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 may play a critical role in heart development. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496 AA; ~85% identity) has recently been identified. It may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology. It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 (50-100ng of control peptide/well)
Western Blot: 1:1000-1:5000 (MW:~55kD)
Optimal dilutions to be determined by the researcher.

Control Peptide (ELISA):
A2293-12

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic 20 amino acid peptide sequence near the N-terminal of mouse Ang-2 (KLH coupled).
Purity:
Neat serum
Form
Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
Specificity:
Recognizes mouse Ang-2. Immunogenic peptide has no significant sequence homology with Ang-1, Ang-3, Ang-4, or ARPs. Species Sequence Homology: rat:100%; porcine: 85%, human:71%; chicken: 55%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Maisonpierre, C., et al., Science 277: 55 (1997). 2. Davis, S., et al., Cell 87: 1161-1169 (1996). 3. Nomura, N., et al., DNA Res. 1: 27-35 (1994). 4. Suri, S., et al., Cell 87: 1171 (1996).