Technical Data
Angiopoietin 2 (Ang-2, AGPT2, ANGPT2, Ang2)
Angiopoietin-1 is an angiogenic secreted protein that interacts with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 may play a critical role in heart development. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496 AA; ~85% identity) has recently been identified. It may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology. It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 (50-100ng of control peptide/well)
Western Blot: 1:1000-1:5000 (MW:~55kD)
Optimal dilutions to be determined by the researcher.

Control Peptide (ELISA):

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Not determined
Synthetic 20 amino acid peptide sequence near the N-terminal of mouse Ang-2 (KLH coupled).
Neat serum
Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
Recognizes mouse Ang-2. Immunogenic peptide has no significant sequence homology with Ang-1, Ang-3, Ang-4, or ARPs. Species Sequence Homology: rat:100%; porcine: 85%, human:71%; chicken: 55%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Maisonpierre, C., et al., Science 277: 55 (1997). 2. Davis, S., et al., Cell 87: 1161-1169 (1996). 3. Nomura, N., et al., DNA Res. 1: 27-35 (1994). 4. Suri, S., et al., Cell 87: 1171 (1996).