Technical Data
A2293-41
Angiopoietin-like 1 (ANGPTL1) (Biotin)
Description:
Angiopoietin-like 1 is a secreted glycoprotein structurally related to the angiopoietins. It contains an N-terminal coiled-coiled domain and a C-terminal fibrinogen-like domain. Unlike angiopoietins which are agonists and antagonists of the Tie-2 receptor tyrosine kinase, Angiopoietin-like 1 is an orphan ligand that does not bind Tie-2.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.2ug/mL Detection limit is ~2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to human Angiopoietin-like 1 expressed in NSO cells (NP_004664).
Purity:
Purified by affinity chromatography
Form
Supplied as a lyophilized powder from PBS, 50ug of BSA. Label with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA
Specificity:
Recognizes human Angiopoietin-like 1. Crossreactivity: rhAngiopoietin-4; <20%, rhAngiopoietin-1 and rhAngiopoietin-2; <1%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.