		Technical Data
A2293-41	Angiopoietin-like 1 (ANGPTL1) (Biotin)

Description:
Produced in goats immunized with purified, NS0-derived, recombinant human Angiopoietin-like 1 (rhAngiopoietin-like 1; Accession # NP_004664). Human Angiopoietin-like 1 specific IgG was purified by human Angiopoietin-like 1 affinity chromatography and then biotinylated. Angiopoietin-like 1 is a secreted glycoprotein structurally related to the angiopoietins. It contains an N-terminal coiled-coiled domain and a C-terminal fibrinogen-like domain. Unlike angiopoietins which are agonists and antagonists of the Tie-2 receptor tyrosine kinase, Angiopoietin-like 1 is an orphan ligand that does not bind Tie-2.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2ug/mL detection limit for rhAngiopoietin-like 1 ~2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Goat

Concentration:

As reported

Immunogen:

NS0-derived recombinant human Angiopoietin-like 1.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder in PBS containing 50ug of BSA. Label with (Biotin) Reconstitute with 200ul 40-50% glycerol, PBS.

Specificity:

Recognizes human Angiopoietin-like 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.