Technical Data
A2293-48D
Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin)
Description:
Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin alphavbeta3. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml Detection limit for rhANGPLT3 is ~1ng/lane under non-reducing and reducing conditions. In this format, A2293-48D shows ~20% cross-reactivity with rmANGPTL3.
Optimal dilutions should be determined by each laboratory for each application.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, TBS, 0.1% BSA. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanSheep
Concentration:
~0.05mg/ml
Immunogen:
Sf21-derived rhANGPLT3 (aa 17-460).
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with 1ml sterile 40-50% glycerol, TBS, 0.1% BSA. Labeled with Biotin.
Specificity:
Recognizes human ANGPLT3. Species sequence homology: 76%, mouse; 83%, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.