Technical Data
A2294-07
Angiostatin
Description:
Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen. Recombinant angiostatin has 260 amino acids, residues 97-357 of human proplasminogen, and a calculated molecular mass of 29.8kD.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Human angiostatin from human plasma.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in 100ul sterile ddH2O.
Specificity:
Recognizes human serum Angiostatin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sim BK, O'Reilly MS, Liang H, Fortier AH, He W, Madsen JW, Lapcevich R, Nacy CA: A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer. Cancer Res Apr;57(7):1329-34 (1997) 2. Shepard SR, Boucher R, Johnston J, Boerner R, Koch G, Madsen JW, Grella D, Sim BK, Schrimsher JL affei M., Halaas J., Ravussin E. et al.: Large-scale purification of recombinant human angiostatin. Protein Expr Purif Nov;20(2):216-27 (2000) 3. Cao Y, Veitonmaki N, Keough K, Cheng H, Lee LS, Zurakowski D: Elevated levels of urine angiostatin and plasminogen/plasmin in cancer patients. Int J Mol Med May;5(5) :547-51 (2000) 4. Ji WR, Barrientos LG, Llinás M, Gray H, Villarreal X, DeFord ME, Castellino FJ, Kramer RA, Trail PA : Selective inhibition by kringle 5 of human plasminogen on endothelial cell migration, an important process in angiogenesis. Biochem Biophys Res Commun Jun;247(2):414-9 (1998)