Technical Data
A2294-07
Angiostatin
Description:
Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen. Recombinant angiostatin has 260 amino acids, residues 97-357 of human proplasminogen, and a calculated molecular mass of 29.8kD.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human angiostatin from human plasma.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human serum Angiostatin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sim BK, O'Reilly MS, Liang H, Fortier AH, He W, Madsen JW, Lapcevich R, Nacy CA: Cancer Res Apr;57(7):1329-34 (1997) 2. Shepard SR, Boucher R, Johnston J, Boerner R, Koch G, Madsen JW, Grella D, Sim BK, Schrimsher JL affei M., Halaas J., Ravussin E. et al.: Protein Expr Purif Nov;20(2):216-27 (2000) 3. Cao Y, Veitonmaki N, Keough K, Cheng H, Lee LS, Zurakowski D: Int J Mol Med May;5(5) :547-51 (2000) 4. Ji WR, Barrientos LG, Llins M, Gray H, Villarreal X, DeFord ME, Castellino FJ, Kramer RA, Trail PA: Biochem Biophys Res Commun Jun;247(2):414-9 (1998)