Technical Data
A2294-48A4
Angiotensin I (Angiotensin-1, Ang I)
Description:
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Immunohistochemistry (PAP or ABC): 1:500
Optimal dilutions to be determined by the researcher.

AA Sequence:
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Angiotensin I.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Specificity:
Recognizes human Angiotensin I. Species crossreactivity: rat, mouse and canine, Salmon [Asn1, Val5, Asn9]-Angiotensin I, bullfrog [Val1, Asn9]-Angiotensin-I, fowl [Val1, Ser9]-Angiotensin-I. Species sequence homology: Goosefish [Asn1, Val5]-Angiotensin-I. Sequence homology (0%): Human Angiotensin II, human Angiotensin III, Endothelin-1 (human, canine, porcine, rat, mouse), a-ANP (aa1-28) (human, canine), human a-Renin Substrate, human BNP-32, [Arg8]-Vasopressin, Substance P, Neurotensin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.