Technical Data
Angiotensin I (Angiotensin-1, Ang I)
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Immunohistochemistry (PAP or ABC): 1:500
Optimal dilutions to be determined by the researcher.

AA Sequence:

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human Angiotensin I.
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Recognizes human Angiotensin I. Species crossreactivity: rat, mouse and canine, Salmon [Asn1, Val5, Asn9]-Angiotensin I, bullfrog [Val1, Asn9]-Angiotensin-I, fowl [Val1, Ser9]-Angiotensin-I. % Cross-reactivity with Peptides: Angiotensin I (Human): 100%; [Asn1, Val5 , Asn 9]-Angiotensin I (Salmon): 100%;[Val1, Asn9]-Angiotensin-I (Bullfrog): 100%; [Val1, Ser9]-Angiotensin-I (Fowl): 100%; [Asn1, Val5]-Angiotensin-I (Goosefish): 13%; Angiotensin II (Human): 0%; Angiotensin III (Human): 0%; Neurotensin: 0%; Substance P: 0%;[Arg8]-Vasopressin: 0%; Endothelin-1 (Human, Canine, Porcine, Rat, Mouse): 0%; BNP-32 (Human): 0%; a-ANP (1-28) (Human, Canine): 0%; Renin Substrate (Human): 0%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.