Technical Data
A2294-98D
Angiotensin Converting Enzyme (ACE, Angiotensin I Converting Enzyme (Peptidyl-dipeptidase A) 1, ACE1, CD143, Dipeptidyl Carboxypeptidase I, DCP1, DCP, Kininase II, MGC26566, MVCD3) (PE)
Description:
CD143 is also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG1i2H5Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Human lung CD143 (Angiotensin converting enzyme).
Purity:
Purified
Form
Supplied as a liquid in 0.09% sodium azide, 1.0% BSA. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human CD143 and active ACE binding to an N-terminal domain epitope different to that recognized by clone.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Danilov, S.M. et al. (1994) Structure - function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806-26814.
2. Metzger, R. et al. (2000) CD143 in the development of atherosclerosis. Atherosclerosis 150: 21-31.