Technical Data
A2295-01T
Angiotensin Converting Enzyme (ACE, CD143)
Description:
Human CD143, also known as angiotensin-converting enzyme (ACE). The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone has been reported to recognize ACE in Western Blot.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and
ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:10-1:100. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended.
Western Blot: 1:50-1:100
Optimal working dilutions to be determined by researcher.

Recommended Positive Control Tissue:
Human Kidney

Recommended Negative Controls:
I1904-79G: Mouse IgG1 Negative Control

Recommended Secondary Reagents:
F(ab')2 rabbit anti-mouse IgG HRP

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H533Supernatant
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Denatured human kidney CD143 (Angiotensin converting enzyme).
Purity:
Tissue culture supernatant.
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The antibody recognizes denatured ACE, binding to an epitope in the C domain. Species Crossreactivity: monkey, rabbit, bovine, canine, feline, guinea pig, goat and sheep. Does not react with porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
. Metzger, R. et al. (1999) Angiotensin-converting enzyme in non-neoplastic kidney diseases Kidney International. 56: 1442 - 1454. 2. Balyasnikova, I. V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens. 61 (1): 49- 62.