Technical Data
A2295-02R3
Angiotensin Converting Enzyme 2 (CT) (ACE 2, ACE2, ACE-2, ACE-related Carboxypeptidase, Angiotensin Converting Enzyme Homolog, ACEH, Angiotensin Converting Enzyme-like Protein, Angiotensin I Converting Enzyme (Peptidyl Dipeptidase A) 2, Angiotensin I Conv
Description:
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently, ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa788-805 of human ACE2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human ACE2, SARS receptor, C-terminal, aa788-805. Does not crossreact with ACE1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Donoghue, M., et al., (2000), “A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9”, Circ. Res., 87, 1-9. 2. Tipnis, S.R., et al., (2000), “A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase”, J. Biol. Chem., 275, 33238–33243. 3. Li, W., et al., (2003), “Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus”, Nature, 426, 450–454. 4. Crackower, M.A., et al., (2002), “Angiotensin-converting enzyme 2 is an essential regulator of heart function” Nature, 417, 822–828.