Technical Data
A2295-05A
Angiotensin II (Angiotensin 2, Ang II)
Description:
Angiotensin is an oligopeptide in the blood that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is a hormone and a powerful dipsogen. It is derived from the precursor molecule angiotensinogen, a serum globulin produced in the liver. It plays an important role in the renin-angiotensin system.
Angiotensin I is converted to angiotensin II through removal of two terminal residues by the enzyme Angiotensin- converting enzyme (ACE, or kinase), which is found predominantly in the capillaries of the lung. ACE is actually found all over the body, but has its highest density in the lung due to the high density of capillary beds there. Angiotensin II acts as an endocrine, autocrine/ paracrine, and intracrine hormone. ACE is a target for inactivation by ACE inhibitor drugs, which decrease the rate of angiotensin II production. Angiotensin II increases blood pressure by stimulating the Gq protein in vascular smooth muscle cells (which in turn activates contraction by an IP3-dependent mechanism). ACE inhibitor drugs are major drugs against hypertension.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1,000-1:10,000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a11C81Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human angiotensin II, DRVYIHPF coupled to a carrier protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Angiotensin II. Due to similarity of angiotensin II and III, antibodies react with both angiotensin II and III but not significantly with angiotensin I or angiotensinogen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Martin MM et al 1995 BBRC 209, 554-562. 2. Chassagne C et al 1995 Genomics 25, 601-603. 3. Koike G ett al 1994 BBRc 203, 1842-50. 4. Tsuzuki S et al 1994 BBRC 200,1449-54. 5. Martin MM et al 1994 BBRC 205, 645-651. 6. Lazard D et al 1994 Receptor Channels 2, 271-280.