Technical Data
A2295-08M
Angiotensin II Type 1 Receptor (AT1)
Description:
The octapeptide circulatory hormone Angiotensin II is a critical regulator of cardiovascular homeostasis formed in blood, with dual function in tissues of the brain, kidney, heart, and vasculature as an autocrine/paracrine growth hormone1,2. The actions of angiotensin II are mediated by type one (AT1) and type two (AT2) angiotensin II receptors, members of the seven-transmembrane G-protein coupled receptor (GPCR) family. AT1 receptors couple predominantly with Gq/11 alpha subunits, and signal through phospholipases C, D, and A2, inositol phosphates, and calcium channels, as well as a variety of serine/threonine and tyrosine kinases2. AT1 expression is greatest in vascular smooth muscle, liver, kidney, adrenal cortex, brain, and lung, and is primarily responsible for the physiological effects of angiotensin II, including regulation of arterial blood pressure, electrolyte and water balance, thirst, hormone secretion, and renal function. The AT2 receptor displays approximately 30% identity to the AT1 receptor, and is highly expressed during embryonic development1,2. AT2 expression in adult tissue is limited to the adrenal medulla, brain, and reproductive tissues, but is upregulated in injured tissue following myocardial infarction, vascular injury, and wound healing. AT2 receptor signaling is not dependent on G- protein coupling or receptor phosphorylation, although coupling to Gia2 and Gia3 alpha subunits occurs in some tissues3. AT2 activation can stimulate tyrosine.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:2000. Predicted Molecular Weight: ~41kD. Higher Molecular Weight species (~55kD) and intermediates also observed which reflect post-translational modification and dimerization of the receptor.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the sequence near the N-terminus of rat angiotensin II receptor AT1. Species Sequence Homology: chimpanzee, bovine, rhesus macaque, sheep, canine, guinea pig, rabbit, equine, wild boar, gerbil.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes rat Angiotensin II Receptor (AT1). Species Crossreactivity: human and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. deGasparo, M., et al. (2000) Pharmacol Rev. 52, 415-472. 2. Griendling, K.K., et al. (1997) Hypertension 29, 366-373. 3. Zhang, J.S., et al. (1996) J Biol Chem. 271, 15026-15033. 4. Siragy, H.M., et al. (2002) J Hypertens. 20, 1157-1163. 5. Gupta, A., et al. (2007) J Biol Chem. 282, 5116-5124. 6. Abdalla, S., et al. (2000) Nature 407, 94-98.