Technical Data
Anion Exchanger 3 (AE3, Anion exchange protein 3, BAE3, brain band 3-like protein, Neuronal band 3-like protein, SLC4A3, Solute carrier family 4 member 3)
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Human AE3 gene (chromosome 2q36) encodes a protein of 1233 aa (mouse/rat 1227 aa). AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml using the control peptide at 1ug/ml.
Western Blot (Chemiluminescence): 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2295-14F2: Anion Exchanger 3, Rat Control Peptide

Storage and Stability
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceRatRabbit
Synthetic peptide corresponding to aa13-32 from rat Anion Exchanger 3 conjugated to KLH. Species Sequence Homology: Mouse, 100%; human, 95%; rabbit, 90%.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Recognizes rat Anion Exchanger 3. No significant sequence homology is detected with other AE or other proteins. Does not recognize cardiac AE3. Epitope: ~N-terminal, cytoplasmic
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kudrycki KE et al (1990) J Biol. Chem. 265: 462. 2. Kopito RR et al (1989) Cell 59: 927. 3. Morgan CW et al (1993) J Cell Sci. 106: 1275. 4. Yannoukakos D et al (1994) Cir. Res. 75: 603. 5. Kopito RR et al (1990) Intl. Rev. Cytol. 123: 177