Technical Data
Ankyrin Repeat Domain 28 (ANKRD28, ANKRD-28, PITK, Phosphatase Interactor Targeting K Protein)
PITK was previously identified as a novel PP1 targeting subunit implicated in modulating the phosphorylation of the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K). Through the phosphorylation of PITK at S1013 and S1017 (residues that flank or reside within a PP1C-binding motif), the binding of the PP1 catalytic subunit to PITK, and subsequently the activity of the holoenzyme, are discretely controlled.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceHumanRabbit
A synthetic peptide mapping to a region between residue 1025 and the C-terminus (residue 1053) of human ankyrin repeat domain 28 (phosphatase interactor targeting K protein) using the numbering given in entry NP_056014.2 (GeneID 23243).
Purified by immunoaffinity chromatography.
Tris-citrate/phosphate buffer, pH 7 to 8
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.