Technical Data
A2295-19P
Ankyrin repeat and SOCS box-containing protein (Asb-2)
Description:
The ankyrin repeat and SOCS box-containing (Asb) protein family is comprised of members which each contain “SOCS boxes” but differ from other families of protein in the motifs they contain upstream of the SOCS box(1). SOCS boxes are carboxy-terminal regions of homology found in the suppressor of cytokine signaling family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. Asb-2 has been found to induce growth inhibition and chromatin condensation in myeloid leukemia cells(2).

Applications:
Western blot analysis of Asb-2 using antibody at 1:1,000 dilution against 15 ug/lane of human spleen lysate (lane 1) and lung lysate (lane 2).

Recommended Dilution:
Western blot: 1:500-1,000

Storage and Stability:
It is supplied as Protein G purified Ig fraction. May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptides containing amino acids 75-93 and 348-368 of human Asb-2 protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Should cross react with mouse and rat Asb-2, based on amino acid sequences used.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kile BT., et al. Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4. Gene. 2000 Nov 27;258(1-2):31-41. 2. Guibal FC., et al. ASB-2 inhibits growth and promotes commitment in myeloid leukemia cells. J Biol Chem. 2002 Jan 4;277(1):218-24. Epub 2001 Oct 26. 3. Turnley AM, Faux CH, Rietze RL, Coonan JR, Bartlett PF. Nat Neurosci 5(11):1155-62 (2002).