Technical Data
Anterior Pharynx Defective 1 Homolog A (APH1A, 6530402N02RIK, APH-1A, APH-1alpha, CGI-78, PSF, UNQ579/PRO1141)
APH1 was initially identified as a component of the Notch pathway in C. elegans (1). Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma- secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid Beta peptide (ABeta) cleavage product that is associated with Alzheimer’s disease (2,3). APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex (4). Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed (5). Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug-20°CBlue IceHumanRabbit
Synthetic peptide, KLH-conjugated. Epitope: 13 amino acid peptide from near the amino terminus of human APH1a.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes Human Anterior Pharynx Defective 1 Homolog A (APH1A). Species Crossreactivity: Human, Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goutte C, et al (2002) Proc. Natl. Acad. Sci. USA. 99:775-9. 2. Periz G, et al (2004) J. Neurosci. Res. 77:309-22. 3. Selkoe DJ (1998) Trends Cell Biol. 8:447-53. 4. Ma G, et al (2005) Neuro. Dis. 25:192-8. 5. Serneels L, et al (2005) Proc.