Technical Data
A2296-83A
Anterior Pharynx Defective 1 Homolog A (APH1A, 6530402N02RIK, APH-1A, APH-1alpha, CGI-78, PSF, UNQ579/PRO1141)
Description:
Anterior Pharynx-Defective 1 (APH1), in addition to presenilin, PEN2, and Nicastrin forms the -secretase protein complex. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. The -secretase complex can cleave the beta-amyloid (A4) precursor protein and yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Three mammalian homologues of APH1 have been identified: APH1a, APH1b, and APH1c, of which APH1a is more abundantly expressed than APH1b in human tissue samples. In addition, two isoforms of APH1a can be generated from alternative splicing of APH-1a, APH1a long form (APH1aL) and APH1a short form (APH1aS) that diverge at the C-terminus. APH1a is the major mammalian APH1 isoform required for Notch signaling during embryogenesis and the major APH1 isoform required for the assembly of the -secretase complexes and APP processing.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-2.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to amino acids 246-265 (RRQEDSRVMVYSALRIPPED) of the C-terminus of human APH-1a conjugated to KLH
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.
Specificity:
Recognizes endogenous levels of human and mouse APH1a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
G Ma. et al. Journal of Neuroscience (2005), 25(1):192-198