Technical Data
A2298-20
Antithrombin (ATIII)
Description:
Antithrombin (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200ug/ml (~3.5uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. The rate of inhibition of thrombin and of activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59kD. Interaction with thrombin results in an SDS-stable binary complex of 96kD.

Applications:
Suitable for use in Neutralization and Clot-based Assays. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Negative Control:
A2298-20A: 100ul purified sheep IgG from non-immune serum for use as negative control. Supplied as a liquid in 0.02M HEPES, pH 7.4, 0.15M sodium chloride, 10mM sodium citrate, 0.02% sodium azide.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanSheep
Concentration:
~25mg/ml
Immunogen:
Human Antithrombin (ATIII).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.02M HEPES, pH 7.4, 0.15M sodium chloride, 10mM sodium citrate, 0.02% sodium azide.
Specificity:
Recognizes human Antithrombin (ATIII). Neutralizes antithrombin in normal plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.