Technical Data
A2298-48C
AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)
Description:
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. SUBUNIT: Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma1/AP1G1 or gamma2/AP1G2 and beta1A/AP1B1 or beta1B/AP1B1), a medium adaptin (mu1A/AP1M1 or mu1B/AP1M2) and a small adaptin (sigma1A/AP1S1 or sigma1B/AP1S2 or sigma1C/AP1S3).

Cellular Localization:
Golgi apparatus. Cytoplasmic vesicle; cytoplasmic vesicle membrane; peripheral membrane protein; cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Alternative Products:
2 named isoforms produced by alternative splicing.

Tissue Specificity:
Widely expressed. DISEASE: Deletion of the AP1B1 gene may play a role in the tumorigenesis of meningiomas. Belongs to the adaptor complexes large subunit family.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide (C-KKGEIFELKAELNSDKKEK) corresponding to the amino acids 11-29 of human AP-1 complex subunit beta-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from A
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity:
This antibody has been shown to be specific for AP-1 complex subunit beta-1. Species Crossreactivity: Rat and human, other species have not yet tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Peyrard M, et al. Genomics 36:112-117(1996). ; 2. Peyrard M, et al. Hum. Mol. Genet. 3:1393-1399(1994). ; 3. Sacksteder C.A, et al. Biochemistry 45:8009-8022(2006). ; 4. Guilbaud C, et al. Mamm. Genome 8:651-656(1997). ; 5. Kirchhausen T, et al. Proc. Natl. Acad. Sci. U.S.A. 86:2612-2616(1989).