Technical Data
A2298-48E
AP1G1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Adaptor Protein Complex AP-1 Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain, Gamma1-adaptin, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, A
Description:
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:8000
Western Blot: 1:500-1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa646-659 of human AP1G1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human AP1G1. Species sequence homology: Chicken, canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.