Technical Data
APC (Adenomatous Polyposis coli)
This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Acetone fixed, frozen sections.
Optimal dilution to be determined by the researcher.

Clinical Relevance:
Detection of APC mutants from colon cell lines.

Positive Control:
Colon cell line HCT116, SW480 and SW837 extracts.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG14i295Affinity Purified
50ug-20CBlue IceHumanMouse
Synthetic peptide corresponding to aa1-433 of the N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular Localization: Cytoplasmic and nuclear.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS. No preservatives added.
Recognizes the N-terminal region of the human APC protein. Epitope Mapping: Performed by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test. Found to bind to APC in the region between aa135-422 (exon 2-3). Species Crossreactivity: Does not crossreact with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Abutaily, A.S., et al.: Cadherins, catenins and APC in pleural malignant mesothelioma. J. Pathol. 201: 355-62 (2003). 2. Fodde, R., et al.: Mutations in the APC tumour suppressor gene cause chromosomal instability. Nat. Cell. Biol. 3: 433-8 (2001). 3. Efstathiou, J.A., et al.: Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells. PNAS USA 95: 3122-7 (1998).