This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Immunohistochemistry: Acetone fixed, frozen sections.
Optimal dilution to be determined by the researcher.
Detection of APC mutants from colon cell lines.
Colon cell line HCT116, SW480 and SW837 extracts.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Synthetic peptide corresponding to aa1-433 of the N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular Localization: Cytoplasmic and nuclear.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in PBS. No preservatives added.|
|Recognizes the N-terminal region of the human APC protein. Epitope Mapping: Performed by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test. Found to bind to APC in the region between aa135-422 (exon 2-3). Species Crossreactivity: Does not crossreact with mouse.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Abutaily, A.S., et al.: Cadherins, catenins and APC in pleural malignant mesothelioma. J. Pathol. 201: 355-62 (2003). 2. Fodde, R., et al.: Mutations in the APC tumour suppressor gene cause chromosomal instability. Nat. Cell. Biol. 3: 433-8 (2001). 3. Efstathiou, J.A., et al.: Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells. PNAS USA 95: 3122-7 (1998).|