Description:
This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry: Acetone fixed, frozen sections.
Optimal dilution to be determined by the researcher.
Clinical Relevance:
Detection of APC mutants from colon cell lines.
Positive Control:
Colon cell line HCT116, SW480 and SW837 extracts.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 4i295 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Synthetic peptide corresponding to aa1-433 of the N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular Localization: Cytoplasmic and nuclear. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS. No preservatives added. |
| Specificity: |
| Recognizes the N-terminal region of the human APC protein. Epitope Mapping: Performed by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test. Found to bind to APC in the region between aa135-422 (exon 2-3). Species Crossreactivity: Does not crossreact with mouse. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Abutaily, A.S., et al.: Cadherins, catenins and APC in pleural malignant mesothelioma. J. Pathol. 201: 355-62 (2003). 2. Fodde, R., et al.: Mutations in the APC tumour suppressor gene cause chromosomal instability. Nat. Cell. Biol. 3: 433-8 (2001). 3. Efstathiou, J.A., et al.: Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells. PNAS USA 95: 3122-7 (1998).
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