Technical Data
APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)
The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme has been shown to function as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1a as well as other transcription factors. Recently, APE has also been shown to control p53 activity through redox alteration. APE is also linked to apoptosis, associated with thioredoxin, and altered levels of APE have been found in some cancers (3). APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control (4).

Suitable for use in Western Blot, Simple Western, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Blocking/Neutralization and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 (ECL). Detects a single band at 37kD.
Simple Western: 1:12.5
Immunohistochemistry (Frozen/Paraffin): 1:100
Immunofluorescence (IC): 1:50-1:200
Immunoprecipitation: 7ug/ml
Chromatin Immunoprecipitation: 1:10-1:500
Blocking/Neutralization: 50-200 molar excess
Optimal dilutions to be determined by researcher.

Positive Control: HeLa whole cell extract

Cellular Localization:
In immunohistochemical experiments punctate nuclear staining is seen in most cell types. However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Affinity purified human APE1 (Uniprot# P27695)
Purified by Immunoaffinity chromatography.
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Recognizes human APE/ref-1. Competitively inhibited from recognizing the APE/ref-1 antigen in tissues using APE/ref-1 protein. Species crossreactivity: mouse, rat, primate, rabbit
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Duguid, J. R., et al., Cancer Res. 55: 6097-102 (1995). 2. Robertson, K.A., et al, Cell Growth Different. 8: 443-449 (1997). 3. Xu, Y., et al, Anticancer Res. 7(5): 3713-3720 (1997). 4. Xanthoudakis, S. et al., Adv. Expt. Med. Biol. 387: 69-75 (1996). 5. Kelley, M.R., et al., Germ Cell Tumors IV. J. Libbey & Co., London,UK, 81-86 (1998). Subject to uses under US Patent No. 5,919,643 (Diagnostic uses) 6,190,661 (Therapeutic uses) 6,406,917