Technical Data
A2298-74A4
APG4A (ATG4A, Autophagy Related Protein 4 Homolog A, Autophagin 2)
Description:
Autophagy related protein 4 homolog A (ATG4A), also known as autophagin 2, is a 50kD cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the autophagin protein family which is involved in autophagy, a biological process in which endogenous proteins and damaged organelles are destroyed. Human ATG4A shares 100%, 94% and 93% identity with that of chimpanzee, dog and mouse.

Applications:
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9H65Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~500ug/ml
Immunogen:
Recombinant human ATG4A insect cell-derived, aa2-398; Q8WYN0.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes human ATG4A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.