Technical Data
A2298-74C7
APEX1 (DNA-(apurinic or apyrimidinic site) lyase, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APEX nuclease, APEN, Protein REF-1, APEX1, APE, APEX, APX, HAP1, REF1)
Description:
Human A (MW: 38kD) endonuclease-1 (HAP-1), also known as APE, APEX or Ref-1, is a nuclear enzyme that repairs apurinic/apyramidinic sites in cellular DNA. Human AP endonucleases-1 cleaves the phosphodiesterase followed by filling-in of the nucleotide gap by DNApolymerase. HAP-1 has been detected in a wide range of human tissues including bladder, breast, small and large intestine, lung, ovary, pancreas, skin, spleen, testis, thymus and thyroid.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2.5-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cells. Tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Recombinant corresponding to human AP endonuclease 1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human APE-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.