Technical Data
APEX1 (DNA-(apurinic or apyrimidinic site) lyase, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APEX nuclease, APEN, Protein REF-1, APEX1, APE, APEX, APX, HAP1, REF1)
Human A (MW: 38kD) endonuclease-1 (HAP-1), also known as APE, APEX or Ref-1, is a nuclear enzyme that repairs apurinic/apyramidinic sites in cellular DNA. Human AP endonucleases-1 cleaves the phosphodiesterase followed by filling-in of the nucleotide gap by DNApolymerase. HAP-1 has been detected in a wide range of human tissues including bladder, breast, small and large intestine, lung, ovary, pancreas, skin, spleen, testis, thymus and thyroid.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2.5-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cells. Tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Recombinant corresponding to human AP endonuclease 1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Recognizes human APE-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.