Technical Data
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
Required for autophagy. SUBUNIT: Conjugated to ATG5.

Cellular Localization:

Alternative Products:
2 named isoforms produced by alternative splicing.

Tissue Specificity:
Ubiquitous. Belongs to the ATG12 family.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
500ug-20CBlue IceHumanRabbit
As reported
A synthetic peptide corresponding to the N-terminal of human APG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to
Purified by affinity chromatography.
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
IHC and WB confirmed the specificity for APG12. Species Crossreactivity: Human, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mizushima, N. et al. J. Biol. Chem. 273(51):33889-33892 (1998). ; 2. Tanida, I. et al. Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002). ; 3. Tanida, I. et al. J. Biol. Chem. 277(16):13739-13744 (2002).