Technical Data
A2298-76P
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
Description:
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG12 is the human homolog of a yeast protein involved in autophagy. APG12 is conjugated to APG5. The APG12-ATG5 conjugate has an apparent molecular mass of 65kD. The C-terminal glycine of APG12 is conjugated to a central lysine (lys130) of APG5. Studies showed that overexpression of APG3 facilitated formation of the APG12-APG5 conjugate, suggesting that APG3 cross-talks with the APG12 conjugation system. APG12 is ubiquitously expressed.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20C. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G313Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Full recombinant human APG12, isoform 2, (1-74) with a GST tag.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human Atg12.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.