Technical Data
A2298-77Z
API5 (Apoptosis Inhibitor 5, API 5, API-5, Antiapoptosis Clone 11 Protein, AAC11, AAC-11, API5-like 1, API5L1, Cell Migration-inducing Gene 8 Protein, Fibroblast Growth Factor 2-interacting Factor 2, FIF, MIG8, Migration Inducing Protein MIG8, Protein XAG
Description:
Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. API5 expression is often upregulated in tumor cells, particularly in metastatic cells. It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1-2ug/ml
Immunohistochemistry (paraffin): 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a sequence at the C-terminal of human API5, identical to the related rat and mouse sequence.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity:
Recognizes human API5. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.