Technical Data
A2298-80A
APJ Receptor (APLNR, Apelin receptor, AGTRL1, angiotensin II receptor-like 1, Angiotensin receptor-like 1, Apelin receptor, APJ, APJR, FLJ90771, G-protein coupled receptor APJ, HG11, MGC45246)
1mg
Molecular Biology Storage: -20°CShipping: Blue Ice
The apelin receptor (APJ receptor) has been identified as the endogenous receptor for the the peptide apelin. Previous to the identification of apelin, APJ was considered an orphan G protein coupled receptor. The apelin receptor (both by immunohistochemistry and in situ) has been localized to the CNS and is currently thought to play a role in stress response, feeding behavior and haemodynamic homeostasis. However, because of a broad distribution the apelin receptor and its endogenous ligand be involved in a wide range of biological process yet to be determined.

Sequence (linear): (Cys)-TIAGHFRKERIEGLRK-amide
See corresponding antibody A2298-80.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Reconstitution Instructions:
The solubility of peptides depends on the primary structure of the molecule. While most dissolve freely in aqueous media, there are many examples of poor solubility, especially when peptides that contain an excess of hydrophobic residues. Unless you know that the peptide is freely soluble in your intended solvent, we recommend the following procedure:
Add a small amount of sterile, distilled water. If the peptide does not dissolve, sonication may help. Alternatively, or subsequently, add a small volume of 10% aqueous acetic acid (for basic peptides) or aqueous ammonia (for acidic peptides). Once the peptide is dissolved, buffer or salt solution may be added to obtain the desired concentration. Sterile filter the final stock solution. Peptides are more stable under acidic conditions and we recommend a choice of pH between 3.0 and 6.0.
If still unable to dissolve:
1. Hydrophobic peptides: Try 1mg in 1ml deionized water and vortex, then add DMF drop by drop and vortex until dissolved.
2. Basic peptides: Try 1% acetic acid solution. If peptide still doesn’t dissolve, add DMF drop by drop and vortex to help dissolve.
3. Acidic peptides: Try 1% ammonium solution. If peptide still doesn’t dissolve, add DMF drop by drop and vortex until dissolved.
Purity: Purified
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.