Technical Data
A2298-80K
APJ Receptor (APLNR, Apelin receptor, AGTRL1, angiotensin II receptor-like 1, Angiotensin receptor-like 1, Apelin receptor, APJ, APJR, FLJ90771, G-protein coupled receptor APJ, HG11, MGC45246)
Description:
The apelin receptor (APJ receptor) has been identified as the endogenous receptor for the the peptide apelin. Previous to the identification of apelin, APJ was considered an orphan G protein coupled receptor. The apelin receptor (both by immunohistochemistry and in situ) has been localized to the CNS and is currently thought to play a role in stress response, feeding behavior and haemodynamic homeostasis. However, because of a broad distribution the apelin receptor and its endogenous ligand be involved in a wide range of biological process yet to be determined.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:10,000-1:50,000
Transfected COS-7 cells, plated on ploy-L-lysine coated glass coverslips, were fixed for 20 min with 4% paraformaldehyde in 0.1M PB, pH 7.4, rinsed with 0.1M TBS, pH 7.4, and preincubated for 30 min at RT with a blocking solution consisting of 5% normal goat serum, 2%BSA, AND 0.1% Triton X-100 in 0.1M TBS. Cells were incubated overnight at 4C with anti-APJ peptide antiserum diluted to 1:10,000-1:50,000 in 0.1M TBS, pH 7.4 containing 1%NGS and 0.05% Triton X-100. After washing with TBS, cells were incubated for 1 hr at RT with appropriate secondary antibody, washed twice with TBS and mounted on glass slides with Aquamount.
Optimal dilutions to be determined by the researcher.

Control Peptides:
A2298-80A, A2298-80B

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
(Cys)-TIAGHFRKERIEGLRK-amide
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human APJ Receptor (Apelin).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.