Technical Data
APJ Receptor (APLNR, Apelin receptor, AGTRL1, angiotensin II receptor-like 1, Angiotensin receptor-like 1, Apelin receptor, APJ, APJR, FLJ90771, G-protein coupled receptor APJ, HG11, MGC45246)
The apelin receptor (APJ receptor) has been identified as the endogenous receptor for the the peptide apelin. Previous to the identification of apelin, APJ was considered an orphan G protein coupled receptor. The apelin receptor (both by immunohistochemistry and in situ) has been localized to the CNS and is currently thought to play a role in stress response, feeding behavior and haemodynamic homeostasis. However, because of a broad distribution the apelin receptor and its endogenous ligand be involved in a wide range of biological process yet to be determined.

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:10,000-1:50,000
Transfected COS-7 cells, plated on ploy-L-lysine coated glass coverslips, were fixed for 20 min with 4% paraformaldehyde in 0.1M PB, pH 7.4, rinsed with 0.1M TBS, pH 7.4, and preincubated for 30 min at RT with a blocking solution consisting of 5% normal goat serum, 2%BSA, AND 0.1% Triton X-100 in 0.1M TBS. Cells were incubated overnight at 4C with anti-APJ peptide antiserum diluted to 1:10,000-1:50,000 in 0.1M TBS, pH 7.4 containing 1%NGS and 0.05% Triton X-100. After washing with TBS, cells were incubated for 1 hr at RT with appropriate secondary antibody, washed twice with TBS and mounted on glass slides with Aquamount.
Optimal dilutions to be determined by the researcher.

Control Peptides:
A2298-80A, A2298-80B

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Recognizes human APJ Receptor (Apelin).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.