Technical Data
A2298-80V2
APLP2 (Amyloid-like Protein 2, Amyloid Protein Homolog, APPH, APPL2, CDEBP, CDEI Box-binding Protein)
Description:
Amyloid beta precursor-like protein 2 (APLP-2) is a 120-170kD member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic Ab peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81%, 93% aa sequence identity with mouse and rat APLP-2, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2B11n11Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa30-680 from human APLP-2 expressed in E. coli (Q06481).
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human APLP-2. Species sequence homology: mouse (81%) and rat (93%). No cross-reactivity with recombinant mouse APLP-2 or recombinant human APLP-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.