Technical Data
Apolipoprotein H (beta 2 Glycoprotein 1, ApoHb2gp1) BioAssay™ ELISA Kit (Antibodies only)
Kits and Assays Storage: -20°CShipping: Blue Ice
Apolipoprotein-H, also known as beta 2-Glycoprotein I (beta 2GPI), is a plasma glycoprotein that circulates at a concentration of 200ug/ml (4uM). Synthesized in the liver, beta 2GPI is a single chain molecule of 48kD, consisting of five repeating internally
disulfide-bonded structures referred to as sushi domains. Relative to other glycoproteins, beta 2GPI has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%). Almost half the circulating beta 2GPI in plasma is associated with lipoproteins of all major fractions. beta 2GPI has been demonstrated to bind negatively charged phospholipids, heparin and platelets. Although the precise function(s) are as yet unknown, beta 2GPI has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage. Recent evidence also implicates beta 2GPI as a cofactor recognized by
anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE).

Materials Supplied:
Antibodies for 5x96 well plates

Kit Components:

2299-81A: Capture Antibody, 1x500ul, Supplied as a liquid in 50% glycerol. Purified by immunoaffinity chromatography. Pab goat x human for coating ELISA plates.

A2299-81B: Detecting Antibody (HRP), 1x500ul, Supplied as a liquid in 50% glycerol.
Purified by immunoaffinity chromatography. Pab goat x human for detection.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Butler JE, Peterman JH, Suter M, Dierks SE; The Immunochemistry of Solid-Phase Sandwich Enzyme-Linked Immunosorbent Assays. Federation Proceedings, 46:8, p 2548-2556, 1987. 2. Harlow E, Lane D; Detecting and Quantitating Antigens using the Two-Antibody Sandwich Assay, in Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, 1988, p 580.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.