Technical Data
APRIL, ED2, Blocking Peptide (a Proliferation Inducing Ligand, TALL-2, TNF and ApoL-related Leukocyte-expressed Ligand 2, TRDL-1a, TNF-related Death Ligand 1a, TNFSF13)
Molecular Biology Storage: -20CShipping: Blue Ice
Synthetic peptide (CIRSMPSHPDRAYNS) corresponding to amino acids 196 to 210 of human APRIL (1-3).

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identi ed by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF-and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1a (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identi ed and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9).

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20C. May be stored at 4C for short-term only. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Synthetic peptide
Purity: Purified 60-70%
Concentration: ~0.2mg/ml
Form: Supplied as a lyophilized powder.

Intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):118590. 2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):6803. 3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):10217. 4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):2526. 5. Wu Y, Bressette D, Carrell JA, et a. Tumor necrosis factor (TNF) receptor superfamily member TACI is a high af nity receptor for TNF family members APRIL and BLyS. J Biol Chem. 2000;275(45):3547885. 6. Marsters SA, Yan M, Pitti RM, et al. Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr Biol. 2000;10(13):7858. 7. Rennert P, Schneider P, Cachero TG, at al. A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth. J Exp Med. 2000;192(11):167784. 8. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease. Nature 2000;404:9959 9. Ware CF. APRIL and BAFF connect autoimmunity and cancer. J Exp Med. 2000;192(11):F358.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.