Technical Data
A2995-10
Aquaporin 0 (AQP0, MIP26)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N- and C-terminus are predicted to be cytoplasmic.

Applications:
Suitable for use in Western Blot, ELISA. Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 (ECL)
ELISA:1:50,000-1:100,000
Immunohistochemistry: Recommend using affinity purified antibody (A2990).
Optimal dilutions to be determined by researcher

Control Peptide: A2995-14

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 17aa within the carboxy terminal domain of human Aquaporin-0. Domain is predicted to be cytoplasmic.
Purity:
Serum
Form
Supplied as a liquid, neat serum, 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes Aquaporin O. Immunizing peptide is unique to AQPO. No significant homology to other AQPs. Species Crossreactivity: Human. Species Sequence Homology: bovine: 94%; rat and mouse: 88%; frog: 70%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pisano, M.M., et al., Genomics 11: 981-990 (1991). 2. Kent, N.A., et al., Nucl. Acid Res. 18: 4256 (1990). 3. Shiels, A., et al. Nucl Acid Res. 16: 9348 (1988). 4. Shiels, A., et al., Nature Genet. 12: 212 (1996).