Technical Data
A3000-11D
Aquaporin 3 (AQP3, GLIP)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the C-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. AQP3 (GLIP, Glycerol-transporting integral protein; 285aa; 31.4kD) has a wide tissue distribution. AQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. In the kidney it is predominantly expressed in the inner medulla. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-termini are predicted to be cytoplasmic.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:100,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:
A3000-11G: Aquaporin 3, Rat, Control Peptide (AQP3, GLIP).
Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to15aa within the cytoplasmic C-terminus of rat AQP3 conjugated to KLH. Species Sequence Homology: mouse; 92%, human, sheep; 85%
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes rat AQP3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishibashi K et al (1994) Proc Natl Acad Sci, 91, 6269-6273; Echevarria M et al (1994) ) Proc Natl Acad Sci, 91, 10997-1100; Ma, TA et al (1994) J Biol. Chem. 269, 21845-21849; 2. Ecelbarger, CA et al (1995) Am. J. Physiol. 269, F663-F672
Websites: http://www.nature.com/labinvest/journal/v82/n11/full/3780560a.html and IF frozen human samples:
http://www.jhc.org/cgi/reprint/47/10/1275.pdf,
http://www.nature.com/jid/journal/v128/n3/full/5701047a.html