Technical Data
A3000-25
Aquaporin 6 (WCH3, hKID, AQP2-like)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP6 (WCH3 or hKID or AQP2-like; 282 aa; 29 kDa; chromosome 12q13; rat 276 aa) is found only in the kidney with low water permeability. Human AQP6 is alternatively spliced to isoform 2, a 163 aa protein that contains 2 extra internal regions, which leads to translations shift and an early termination, as compared to the 282- aa isoform 1. the isoform 2 contains an identical N-terminal 134-aa and a distinct 29-aa C-terminus. Human AQP6 AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%).

Applications:
Suitable for use in ELISA, Western Blot. and Immunofluorescence Other appications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug.mlusing control peptide (Cat #A3000-25D) to coat plates to 1ug/ml.
Western Blot: 1-10ug/ml (ECL)
Immunofluorescence: 2-10ug/ml in paraformaldehyde fixed sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
A synthetic peptide corresponding to 19aa near the C-terminal, cytoplamic domain of human Aquaporin 6.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human Aquaporin 6. Species Sequence Homology: rat, 47%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
M1. Ma T (1996) Genomics 35, 543-550; Ma T (1997) genomics 43, 387; 2. Yasui M (1999) PNAS 96, 5808. 3. Jeremic A, 2005, Exp. Biol. Med. 230: 674 - 680. 4. Wang W 2003 Calcified T Intl. 72(3):222-7