Technical Data
Aquaporin 9, Rat, Control Peptide (AQP9)
Molecular Biology Storage: -20CShipping: Blue Ice
Control Peptide for A3009-45 (antiserum) and A3009-40 (affinity purified)

An 18aa synthetic peptide within the C-terminal domain of rat AQP9.

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.

A new member of AQP family, AQP9, has been cloned form human leukocytes by homology cloning (1). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.

Recommended Dilution:
Antibody Blocking: 5-10ug per 1ul A3009-45 (antiserum) or per 1ug A3009-40 (affinity purified antibody).
ELISA: 50-100ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Rat synthetic peptide
Purity: Highly purified
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide
Specificity: Unique to AQP9 without significant homology to any other AQPs. It has 72% homology with the hAQP9. AQP9 from other species are yet to be cloned. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Koyama, Yu, et al., JBC 272: 30329 (1997). 2. Ishibashi, K., et al., BBRC 714: 714-718 (1997). 3. Okada, S.: FEBS Letters 540: 157-162 (2003). 4. Barcroft, L.C.: Developmental Biology 256: 342-354 (2003). 5. Hubert, C., et al.: J. Biol. Chem 277: 22710-22717 (2002). 6. Liu, Z., et al,: PNAS 99: 6053-6058 (2002). 7. Nicchia, G.P.: J. Histochem. Cytochem. 49: 1547-1556 (2001). 8. Badaut, J.: Neuroscience (2004). 9. Liu, Z.: BBRC 316: 1178-1185 (2004). 10. Wang, Am. J. of Ob. Gynecol. 191: 2160 (2002). 11. Kenney, J. Histochem. Cytochem. 52: 1341-1350 (2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.