Technical Data
Aquaporin 11 (AQP11, AQP-11, AQPX1, PSEC0027, Aquaporin-11)
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, AQP11, has been cloned. AQP11 (human, rat mouse: 271aa each).

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml. Conrtol peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 2-10ug/ml in paraformaldehyde fixed sections of tissues
Optimal dilutions to be determined by the researcher.

Conrtol Peptide:
A3000-85B: Aquaporin 11, Control Peptide (AQP11, AQP-11, AQPX1, PSEC0027, Aquaporin-11)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
~1mg/ml (after reconstitution)
Two synthetic peptides corresponding to mouse and human AQP11 near the C-terminal, conjugated to KLH. (UniProt #s mouse Q8BHH1 and human Q8NBQ7).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Recognizes AQP11. Species Sequence Homology: mouse, rat and human, 84%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishibashi, K., M. et al. (2000). p. 123- 126. In S. Hohmann and S. Nielsen (ed.), Molecular biology and physiology of water and solute transport. Kluwer Academic/Plenum Publishers, New York, N.Y.