Technical Data
A3000B
Aquaporin 1 (AQP1, CHIP28) (Azide, BSA & Glycerol free)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (Rat kidney membranes): 1:400-1:800
Immunohistochemistry: Rat kidney sections.
Optimal dilution determined by the researcher.

Control Antigen:
A3000A: Aquaporin 1 (AQP1, CHIP28), Control Antigen

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommended adding glycerol as a cryopreservative.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4°C Do Not FreezeBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa243-261 of human AQP1 (KVWTS GQVEE YDLDA DDIN). SwissProt accession number P29972. Epitope location: Intracellular, C-terminus.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol free.
Specificity:
Recognizes Aquaporin 1. Species crossreactivity: Rat and human. Species Sequence Homology: Rat, mouse, bovine, sheep: 100%; Canine: 18/19; Frog (Rana esculenta), toad (Bufo marinus): 14/19.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Quigley, R. et al. (1998) J. Membr. Biol. 164, 177. 2. Mittal, A. et al. (2000) Life Sci., 66, 1471.