Technical Data
A3000C
Aquaporin 1 (AQP1, CHIP28) (Biotin) (Azide, BSA & Glycerol free)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (Rat kidney membranes): 1:400-1:800
Immunohistochemistry: Rat kidney sections.
Optimal dilution determined by the researcher.

Control Antigen:
A3000A: Aquaporin 1 (AQP1, CHIP28), Control Antigen

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4°C (-20°C Reconstitution)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa243-261 of human AQP1 (KVWTS GQVEE YDLDA DDIN). SwissProt accession number P29972. Epitope location: Intracellular, C-terminus.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol free. Conjugated to Biotin.
Specificity:
Recognizes Aquaporin 1. Species crossreactivity: Rat and human. Species Sequence Homology: Rat, mouse, bovine, sheep: 100%; Canine: 18/19; Frog (Rana esculenta), toad (Bufo marinus): 14/19.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Quigley, R. et al. (1998) J. Membr. Biol. 164, 177. 2. Mittal, A. et al. (2000) Life Sci., 66, 1471.