Technical Data
ARAP2 (Arf-GAP with Rho-GAP Domain, ANK Repeat and PH Domain-containing Protein 2, Centaurin-delta-1, Cnt-d1, Protein PARX, CENTD1, KIAA0580)
ARAP2, also known as Centaurin-delta-1, is a protein with ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. A phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein, ARAP2 binds to RhoA-GTP, but lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. It modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Usually ARAP2 is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding and can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. ARAP2 associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. It is usually detected in brain, thymus, lymph node, thyroid, spinal cord, trachea, heart, skeletal muscle, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa78-93 ANVHKTKKNDDPSKDY of human ARAP2.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human ARAP2. Species Crossreactivity: chimpanzee. Species sequence homology: equine, rhesus monkey, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nagase, T. et al. DNA Res. 5:31-39 (1998). 2. Miura, K. et al. Molec. Cell. 9:109-119 (2002).