Technical Data
A3330-04AX
ARF, p14 (p16beta) (BSA & Azide Free)
Description:
The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 protein required for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1beta-encoded Nterminal domain of p14ARF and a C-terminal region of mdm2. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways.

Applications:
Suitable for use in Immunofluorescence, Western Blot and
Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 5-10ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 5-10ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells, cervical carcinoma or breast fibroadenoma.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide from the C-terminus of human p14ARF. Cellular Localization: Nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A3330-04A
Specificity:
May show some additional bands with MW much higher than 14kD, however, the p14ARF area is relatively clean which permits an easy identification of the p14ARF band. For optimun results, immunoprecipitate p14ARF with Western blot. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang Y, et al. Cell, 92: 725734, 1998