Technical Data
A3340-35
Arg, SH3/SH2 (Tyrosine Kinase Abl-related Gene)
Description:
Applications:
Suitable for use in Western Blot., Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000 detects Arg in RIPA lysates from mouse brain cytosol. Mouse brain cytosol was resolved by electrophoresis, transferred to nitrocellulose and probed with A3340-35. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: Reported to detect Arg, in either paraf n-embedded or frozen murine brain sections.
Immunoprecipitation: Reported to immunoprecipitate enzymatically active Arg from 100ug mouse brain RIPA lysates.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceMouseRabbit
Concentration:
Not Determined
Immunogen:
GST fusion protein corresponding to the SH2 and SH3 domains of murine Arg (Abl-related gene), residues 112-259. Species Sequence Homology: human and Xenopus .
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity:
Recognizes murine Arg, doublet, Mr 135 and 140kD. Two non-speci c proteins were also detected at ~Mr 170 and 250kD
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cao, C., et al. J. of Biol. Chem. 276: 11465-11468, 2000. 2. Koleske, A. J., et al. Neuron 21: 1259-1272, 1998.